Collection: Anionic Liposomes

Anionic liposomes, a subtype of liposomes characterized by their negatively charged lipid bilayers, have garnered significant attention in the field of drug delivery, gene therapy, vaccines, and imaging due to their unique properties and versatile applications. This comprehensive review aims to provide a thorough understanding of anionic liposomes, covering their composition, preparation methods, physicochemical properties, characterization techniques, applications, challenges, and future directions. By examining the latest research findings and technological advancements, this review seeks to contribute to the current knowledge base and stimulate further exploration in this promising area of nanomedicine.

  1. Introduction:

   Liposomes, spherical vesicles composed of lipid bilayers, have emerged as promising vehicles for the delivery of therapeutic agents, owing to their biocompatibility, versatility, and ability to encapsulate a wide range of molecules. Among different types of liposomes, anionic liposomes stand out due to the presence of negatively charged lipids in their composition. The negatively charged surface of anionic liposomes imparts unique properties that can be harnessed for various biomedical applications.

  1. Composition and Preparation Methods:

   Anionic liposomes are typically composed of lipids containing negatively charged head groups, such as phosphatidylserine (PS) or phosphatidic acid (PA), along with neutral or zwitterionic lipids. Various methods are employed for the preparation of anionic liposomes, including thin-film hydration, reverse-phase evaporation, and microfluidic techniques. The choice of lipid composition and preparation method influences the size, surface charge, and stability of the resulting liposomes.

  1. Physicochemical Properties and Characterization Techniques:

   Anionic liposomes exhibit distinct physicochemical properties, including size, surface charge, membrane fluidity, and stability, which play crucial roles in their biological interactions and therapeutic efficacy. Characterization techniques such as dynamic light scattering, zeta potential analysis, transmission electron microscopy, and nuclear magnetic resonance spectroscopy are employed to elucidate the structural and functional properties of anionic liposomes.

  1. Applications of Anionic Liposomes:

   Anionic liposomes have found widespread applications in drug delivery, gene therapy, vaccines, imaging, and cosmetics. In drug delivery, anionic liposomes offer advantages such as targeted delivery, controlled release, and improved pharmacokinetics. They are also utilized as non-viral vectors for gene delivery, enabling the transport of nucleic acids into target cells for gene therapy applications. Additionally, anionic liposomes serve as efficient carriers for vaccine antigens, enhancing immunogenicity and enabling the development of novel vaccine formulations. Moreover, they are employed as contrast agents in various imaging modalities, facilitating the visualization of tissues and organs for diagnostic purposes. In the cosmetics industry, anionic liposomes are incorporated into skincare products for the efficient delivery of active ingredients, enhancing their efficacy and bioavailability.


  1. Challenges and Future Directions:

   Despite their promising applications, anionic liposomes face challenges related to stability, biocompatibility, and scalability. Strategies such as surface modification, encapsulation of stabilizing agents, and optimization of formulation parameters are being explored to overcome these challenges. Future research directions include the development of multifunctional anionic liposomes capable of targeted delivery, stimuli-responsive release, and theranostic applications. Additionally, advancements in nanotechnology and material science hold promise for the design of novel lipid-based nanostructures with enhanced properties and functionalities.

  1. Conclusion:

   Anionic liposomes represent a versatile platform for the delivery of therapeutic agents in various biomedical applications. Their unique properties, including negatively charged surfaces, make them attractive candidates for targeted drug delivery, gene therapy, vaccines, imaging, and cosmetics. By addressing challenges related to stability and biocompatibility, and by exploring innovative strategies for functionalization and modulation, anionic liposomes hold significant potential for revolutionizing drug delivery and healthcare technologies.


This comprehensive review provides a holistic understanding of anionic liposomes, encompassing their composition, properties, preparation methods, applications, challenges, and future directions. By synthesizing the latest research findings and technological advancements, this review aims to inspire further exploration and innovation in the field of lipid-based nanomedicine.