General Information
Cationic DOTAP-Based Tracer Liposomes are specialized liposomal formulations that utilize the cationic lipid DOTAP (1,2-dioleoyl-3-trimethylammonium-propane) for the efficient tracking and tracing of liposomes in biological systems. These liposomes are labeled with detectable tracers, such as fluorescent dyes or radioactive isotopes, to monitor their distribution, uptake, and biodistribution in vivo and in vitro.
Applications
These tracer liposomes are particularly effective in drug delivery research, gene therapy studies, pharmacokinetic analyses, and more. The cationic nature of DOTAP enhances cellular uptake, making these liposomes ideal for studies that require efficient delivery and tracking within biological systems.
Benefits
Cationic DOTAP-Based Tracer Liposomes provide real-time insights into the distribution and behavior of liposomes in biological systems, offering valuable data on the efficiency of delivery systems. This technology is critical for the development and optimization of targeted therapies and gene delivery applications.
Materials Needed:
- Cationic DOTAP-Based Tracer Liposomes (labeled with desired tracer)
- Phosphate-buffered saline (PBS) for dilution
- Dynamic Light Scattering (DLS) instrument for particle size analysis
- Fluorescence or radioactivity detection equipment
- Refrigeration storage (4°C)
- Gloves and appropriate PPE
Procedure:
1. Preparation:
- Ensure that the Cationic DOTAP-Based Tracer Liposomes are stored at the recommended temperature (typically 4°C) before use.
- Prepare the necessary dosage based on the experimental or clinical requirements.
2. Dilution:
- Prior to administration or experimental use, dilute the liposomes in PBS to the desired concentration. Use sterile techniques to avoid contamination.
3. Administration (for research purposes):
- Administer the diluted Tracer Liposomes to the appropriate cell culture or animal model. The dosage and method of administration will depend on the experimental design.
4. Detection and Analysis:
- Use appropriate detection equipment (e.g., fluorescence microscopy, gamma counter) to monitor the distribution and uptake of the Cationic DOTAP-Based Tracer Liposomes in the biological system.
5. Quality Control:
- Assess the stability and integrity of the Tracer Liposomes using DLS and other relevant techniques to ensure accurate tracing results.
6. Storage:
- Store any remaining Tracer Liposomes at 4°C. Avoid repeated freeze-thaw cycles to maintain stability and tracer integrity.
Specifications
| Specification | Details |
|---|---|
| Lipid Composition | DOTAP:DOPC:Cholesterol (5:65:30) |
| Liposomal Size | 100-150 nm (can be customized) |
| Tracer Type | Fluorescent dye, radioactive isotope, or other markers |
| Storage Temperature | 4°C |
| Usage | Single-use, research and clinical applications |
| Applications | Biodistribution studies, drug delivery research, gene therapy, imaging |
Cationic DOTAP-Based Tracer Liposomes are designed for the efficient tracking and tracing of liposomes in biological systems. The cationic nature of DOTAP facilitates strong interactions with negatively charged cellular membranes, enhancing the uptake and intracellular delivery of the liposomes. By labeling these liposomes with fluorescent dyes or radioactive tracers, researchers can monitor their distribution, uptake, and efficacy in real-time, providing critical data for the development of gene therapies and targeted drug delivery systems.
Apurun ensures secure shipping of Cationic DOTAP-Based Tracer Liposomes, with temperature-controlled packaging to maintain product integrity. Standard shipping times apply, with options for expedited delivery available upon request.
Store Cationic DOTAP-Based Tracer Liposomes at 4°C. Protect from light (if labeled with fluorescent tracers) and avoid repeated freeze-thaw cycles to maintain efficacy. Proper handling procedures should be followed to ensure safety and product quality.
