General Information
The NanoSizer Mini Extruder is the world's first single-use, fully assembled extruder designed to simplify the process of lipid extrusion. This innovative tool eliminates the need for assembly and cleaning, reducing the risk of contamination and saving valuable time in the laboratory.
Revolutionary Design
The NanoSizer Mini Extruder offers unmatched convenience and reliability. Its fully assembled, single-use design ensures that each extrusion process is free from contamination risks, making it an ideal choice for researchers who require consistent and reproducible results. This design innovation means no more laborious setup or potential cross-contamination from previous uses.
Ease of Use
Using the NanoSizer Mini Extruder is straightforward. Simply load your sample into the extruder, select the appropriate filter size, and initiate the extrusion process. The pre-assembled unit requires no additional setup, allowing researchers to focus more on their experiments and less on the preparation of equipment.
Technical Specifications
The NanoSizer Mini Extruder is available in various filter sizes, ranging from 50 nm to 200 nm, to accommodate different experimental needs. Each unit is designed to deliver consistent performance, ensuring uniform particle size distribution with minimal effort. This flexibility allows it to be used in a wide range of applications, from drug delivery to nanomedicine.
Materials Needed:
- Lipids (e.g., phospholipids such as phosphatidylcholine)
- Organic solvent (e.g., chloroform or a suitable solvent)
- Buffer solution (for hydration)
- Mini extruder with appropriate membrane filters (typically with pore sizes between 50 nm and 200 nm)
- Vacuum pump (optional, for degassing)
- Sonicator (optional, for further size reduction)
- Glass vials or containers
- Syringe and needles
- Graduated cylinders and pipettes
- Dialysis tubing (if needed for purification)
- SpeedVac concentrator (optional, for solvent removal)
Procedure:
1. Lipid Film Formation:
- Dissolve the lipids in a suitable organic solvent (e.g., chloroform) to create a lipid solution. The lipid concentration will depend on your specific formulation.
- Evaporate the solvent using a rotary evaporator, a stream of nitrogen gas, or a SpeedVac concentrator to form a thin lipid film on the walls of a glass container. Ensure that the film is evenly distributed.
2. Hydration:
- Add an appropriate buffer solution to hydrate the lipid film. The buffer composition can vary based on the intended application.
- Swirl or vortex the container gently to help the lipids hydrate, forming multilamellar vesicles (MLVs).
3. Probe Sonication (Optional):
- If desired, perform probe sonication to further break down the MLVs into smaller liposomes. Be cautious not to overheat the sample during sonication.
4. Extrusion Setup:
- Assemble the mini extruder with a membrane filter of the desired pore size. Ensure that the extruder is clean and free from air bubbles.
5. Loading the Sample:
- Load the hydrated liposome suspension into a syringe and attach it to the extruder.
6. Extrusion Passes:
- Extrude the liposome suspension through the membrane filter for a total of 11 passes. For each pass, use the syringe to push the suspension through the filter.
7. Collecting Liposomes:
- Collect the liposome suspension in a clean container after each pass. You will notice a reduction in size and an increase in homogeneity with each pass.
8. Quality Control:
- Measure the size distribution and polydispersity index (PDI) of the liposomes using dynamic light scattering (DLS) or another appropriate characterization method. This will help you determine if further passes are necessary.
9. Additional Passes (if needed):
- If the liposomes do not meet the desired size and uniformity after 11 passes, you can perform additional passes until the desired characteristics are achieved.
10. Purification (Optional):
- If required, purify the liposomes by dialysis against the buffer solution to remove any remaining solvent or unencapsulated materials.
11. Storage:
- Store the unilamellar liposomes in appropriate conditions (e.g., refrigeration or freeze-drying) and use them for your intended application.
General Information
The NanoSizer Mini Extruder is the world's first single-use, fully assembled extruder designed to simplify the process of lipid extrusion. This innovative tool eliminates the need for assembly and cleaning, reducing the risk of contamination and saving valuable time in the laboratory.
Revolutionary Design
The NanoSizer Mini Extruder offers unmatched convenience and reliability. Its fully assembled, single-use design ensures that each extrusion process is free from contamination risks, making it an ideal choice for researchers who require consistent and reproducible results. This design innovation means no more laborious setup or potential cross-contamination from previous uses.
Ease of Use
Using the NanoSizer Mini Extruder is straightforward. Simply load your sample into the extruder, select the appropriate filter size, and initiate the extrusion process. The pre-assembled unit requires no additional setup, allowing researchers to focus more on their experiments and less on the preparation of equipment.
Specifications
| Specification | Details |
|---|---|
| Filter Sizes | 50 nm, 100 nm, 200 nm |
| Material | High-quality, biocompatible materials |
| Usage | Single-use, pre-assembled |
| Compatibility | Wide range of lipid formulations |
| Applications | Drug delivery, nanomedicine, research |
| Package Contents | Fully assembled extruder, instructions |
This is the content for line 4.
This is the content for line 5.
Storage and Handling
- Choosing a selection results in a full page refresh.
- Opens in a new window.