General Information
Cationic & PEGylated-Based Lyophilized Liposomes are specialized liposomal formulations that incorporate cationic lipids combined with polyethylene glycol (PEG). These liposomes have undergone lyophilization (freeze-drying), allowing them to be stored in a stable, dry form and reconstituted when needed. The cationic charge aids in the interaction with negatively charged molecules, such as DNA and RNA, while PEGylation enhances the stability, circulation time, and reduces immunogenicity of the liposomes, making them ideal for gene delivery, drug delivery, and other therapeutic applications.
Applications
Cationic & PEGylated Liposomes are extensively used in pharmaceuticals, particularly in gene therapy, drug delivery systems, and therapeutic research. Their lyophilized form ensures long-term stability, making them convenient for storage and transport. Upon reconstitution, these liposomes can be utilized in both in vitro and in vivo studies, providing an effective delivery system for nucleic acids, peptides, and proteins.
Benefits
These liposomes offer the benefits of extended shelf life and stability due to lyophilization, combined with the enhanced circulation time and reduced immunogenicity provided by PEGylation. The cationic nature facilitates interaction with negatively charged molecules, making these liposomes particularly suitable for delivering genetic material or charged therapeutic agents.
Materials Needed:
- Cationic & PEGylated-Based Lyophilized Liposomes
- Sterile water or buffer solution for reconstitution
- Phosphate-buffered saline (PBS) for further dilution
- Dynamic Light Scattering (DLS) instrument for particle size analysis
- Refrigeration storage (2-8°C)
- Gloves and appropriate PPE
Procedure:
1. Reconstitution:
- Add sterile water or buffer solution to the lyophilized liposomes. The volume of liquid should be based on the desired final concentration.
- Gently swirl or vortex the vial until the liposomes are fully reconstituted. Avoid vigorous shaking to prevent foaming or damage to the liposomes.
2. Dilution:
- After reconstitution, further dilute the liposomes in PBS if necessary to achieve the desired working concentration.
3. Administration (for research purposes):
- Administer the reconstituted liposomes to the appropriate cell culture or animal model. The dosage and method of administration will depend on the experimental design.
4. Quality Control:
- Use DLS and other analytical methods to confirm the size and stability of the reconstituted liposomes. Ensure that the liposomal structure has been preserved post-lyophilization.
5. Storage:
- Store any remaining reconstituted liposomes at 2-8°C. For long-term storage, it is recommended to keep the liposomes in their lyophilized form.
Specifications
| Specification | Details |
|---|---|
| Lipid Composition | Cationic Lipids:Cholesterol:PEG (Varies depending on formulation) |
| Liposomal Size | 100-150 nm (can be customized) |
| Lyophilization Process | Freeze-drying with cryoprotectants to preserve structure |
| Storage Temperature | 2-8°C for reconstituted, room temperature for lyophilized form |
| Usage | Single-use, research and therapeutic applications |
| Applications | Gene delivery, drug delivery, therapeutic research, nucleic acid delivery |
Cationic & PEGylated-Based Lyophilized Liposomes are engineered for applications where enhanced circulation time, reduced immunogenicity, and specific interactions with negatively charged molecules are critical. The cationic charge aids in targeting negatively charged genetic material, while PEGylation provides a steric barrier that prevents rapid clearance by the reticuloendothelial system (RES), allowing these liposomes to remain in circulation longer. These properties are particularly advantageous in gene therapy and nucleic acid delivery, where stability and prolonged action are crucial. The lyophilization process ensures that these liposomes retain their structural integrity over time, providing a stable and reliable delivery system.
Apurun ensures secure shipping of Cationic & PEGylated-Based Lyophilized Liposomes, with temperature-controlled packaging to maintain product integrity. Lyophilized liposomes can be shipped at ambient temperatures, while reconstituted liposomes should be shipped with cold packs. Standard shipping times apply, with options for expedited delivery available upon request.
Store Cationic & PEGylated-Based Lyophilized Liposomes at room temperature in their lyophilized form. After reconstitution, store the liposomes at 2-8°C. Protect from light and avoid repeated freeze-thaw cycles to maintain efficacy. Proper handling procedures should be followed to ensure safety and product quality.
