General Information
Diether-Based Lyophilized Liposomes are specialized liposomal formulations that incorporate diether lipids. These liposomes have undergone lyophilization (freeze-drying), allowing them to be stored in a stable, dry form and reconstituted when needed. Diether lipids are known for their stability and resistance to oxidative degradation, making these liposomes particularly useful in applications requiring long-term stability and integrity, such as drug delivery and vaccine development.
Applications
Diether-Based Lyophilized Liposomes are used in a variety of research and clinical fields, including drug delivery, vaccine formulation, and therapeutic research. Their lyophilized form ensures long-term stability, making them convenient for storage and transport. Upon reconstitution, these liposomes can be used in both in vitro and in vivo experiments, providing a stable and effective delivery system for various bioactive compounds.
Benefits
These liposomes offer the benefits of extended shelf life and stability due to lyophilization, combined with the superior oxidative stability of diether lipids. This makes them an excellent choice for delivering sensitive therapeutic agents and for applications where long-term storage is required without compromising the liposomal integrity.
Materials Needed:
- Diether-Based Lyophilized Liposomes
- Sterile water or buffer solution for reconstitution
- Phosphate-buffered saline (PBS) for further dilution
- Dynamic Light Scattering (DLS) instrument for particle size analysis
- Refrigeration storage (2-8°C)
- Gloves and appropriate PPE
Procedure:
1. Reconstitution:
- Add sterile water or buffer solution to the lyophilized liposomes. The volume of liquid should be based on the desired final concentration.
- Gently swirl or vortex the vial until the liposomes are fully reconstituted. Avoid vigorous shaking to prevent foaming or damage to the liposomes.
2. Dilution:
- After reconstitution, further dilute the liposomes in PBS if necessary to achieve the desired working concentration.
3. Administration (for research purposes):
- Administer the reconstituted liposomes to the appropriate cell culture or animal model. The dosage and method of administration will depend on the experimental design.
4. Quality Control:
- Use DLS and other analytical methods to confirm the size and stability of the reconstituted liposomes. Ensure that the liposomal structure has been preserved post-lyophilization.
5. Storage:
- Store any remaining reconstituted liposomes at 2-8°C. For long-term storage, it is recommended to keep the liposomes in their lyophilized form.
Specifications
| Specification | Details |
|---|---|
| Lipid Composition | Diether Lipids:Cholesterol (50:50) |
| Liposomal Size | 100-150 nm (can be customized) |
| Lyophilization Process | Freeze-drying with cryoprotectants to preserve structure |
| Storage Temperature | 2-8°C for reconstituted, room temperature for lyophilized form |
| Usage | Single-use, research and clinical applications |
| Applications | Drug delivery, vaccine formulation, therapeutic research |
Diether-Based Lyophilized Liposomes are designed for applications requiring long-term stability and resistance to oxidative stress. The inclusion of diether lipids, which are known for their resistance to oxidative degradation, makes these liposomes particularly suitable for the delivery of sensitive therapeutic agents and for use in environments where oxidative damage is a concern. Upon reconstitution, these liposomes retain their original size and characteristics, providing consistent performance in research and therapeutic applications. The lyophilization process further enhances their shelf life, making them a reliable option for long-term storage.
Apurun ensures secure shipping of Diether-Based Lyophilized Liposomes, with temperature-controlled packaging to maintain product integrity. Lyophilized liposomes can be shipped at ambient temperatures, while reconstituted liposomes should be shipped with cold packs. Standard shipping times apply, with options for expedited delivery available upon request.
Store Diether-Based Lyophilized Liposomes at room temperature in their lyophilized form. After reconstitution, store the liposomes at 2-8°C. Protect from light and avoid repeated freeze-thaw cycles to maintain efficacy. Proper handling procedures should be followed to ensure safety and product quality.
