General Information
DOTAP-Based Lyophilized Liposomes are specialized liposomal formulations that incorporate the cationic lipid DOTAP (1,2-dioleoyl-3-trimethylammonium-propane). These liposomes have undergone lyophilization (freeze-drying), allowing them to be stored in a stable, dry form and reconstituted when needed. DOTAP is widely used for gene delivery, RNA/DNA transfection, and targeted drug delivery due to its positive charge, which facilitates interactions with negatively charged nucleic acids and cell membranes.
Applications
DOTAP-Based Lyophilized Liposomes are highly effective in fields such as gene therapy, vaccine development, and molecular biology research. Their lyophilized form ensures long-term stability, making them convenient for storage and transport. Upon reconstitution, these liposomes can be used in both in vitro and in vivo applications, including transfection studies, gene delivery, and targeted drug delivery.
Benefits
These liposomes offer the benefits of extended shelf life and stability due to lyophilization, along with the functional properties of DOTAP for efficient delivery of nucleic acids and therapeutic agents. The positive charge of DOTAP enhances cellular uptake and interaction with nucleic acids, making these liposomes a powerful tool in gene therapy and transfection applications.
Materials Needed:
- DOTAP-Based Lyophilized Liposomes
- Sterile water or buffer solution for reconstitution
- Phosphate-buffered saline (PBS) for further dilution
- Dynamic Light Scattering (DLS) instrument for particle size analysis
- Refrigeration storage (2-8°C)
- Gloves and appropriate PPE
Procedure:
1. Reconstitution:
- Add sterile water or buffer solution to the lyophilized liposomes. The volume of liquid should be based on the desired final concentration.
- Gently swirl or vortex the vial until the liposomes are fully reconstituted. Avoid vigorous shaking to prevent foaming or damage to the liposomes.
2. Dilution:
- After reconstitution, further dilute the liposomes in PBS if necessary to achieve the desired working concentration.
3. Administration (for research purposes):
- Administer the reconstituted liposomes to the appropriate cell culture or animal model. The dosage and method of administration will depend on the experimental design.
4. Quality Control:
- Use DLS and other analytical methods to confirm the size and stability of the reconstituted liposomes. Ensure that the liposomal structure has been preserved post-lyophilization.
5. Storage:
- Store any remaining reconstituted liposomes at 2-8°C. For long-term storage, it is recommended to keep the liposomes in their lyophilized form.
Specifications
| Specification | Details |
|---|---|
| Lipid Composition | DOTAP:DOPC:Cholesterol (50:30:20) |
| Liposomal Size | 100-150 nm (can be customized) |
| Lyophilization Process | Freeze-drying with cryoprotectants to preserve structure |
| Storage Temperature | 2-8°C for reconstituted, room temperature for lyophilized form |
| Usage | Single-use, research and clinical applications |
| Applications | Gene delivery, transfection studies, RNA/DNA delivery, targeted drug delivery |
DOTAP-Based Lyophilized Liposomes are engineered for efficient delivery of nucleic acids and other therapeutic agents. DOTAP's positive charge enhances its interaction with negatively charged molecules like DNA and RNA, facilitating cellular uptake and transfection. The lyophilization process ensures that these liposomes maintain their integrity and functionality over time, making them ideal for long-term storage and use in gene therapy and molecular biology applications. Upon reconstitution, these liposomes retain their original size and charge properties, providing consistent performance in research and therapeutic studies.
Apurun ensures secure shipping of DOTAP-Based Lyophilized Liposomes, with temperature-controlled packaging to maintain product integrity. Lyophilized liposomes can be shipped at ambient temperatures, while reconstituted liposomes should be shipped with cold packs. Standard shipping times apply, with options for expedited delivery available upon request.
Store DOTAP-Based Lyophilized Liposomes at room temperature in their lyophilized form. After reconstitution, store the liposomes at 2-8°C. Protect from light and avoid repeated freeze-thaw cycles to maintain efficacy. Proper handling procedures should be followed to ensure safety and product quality.
