General Information
Doxorubicin Liposomes - Azide (PEGylated) are advanced drug delivery systems designed for targeted cancer therapy. The azide functional group attached to the PEGylated liposomes allows for bioorthogonal click chemistry, enabling precise attachment of targeting ligands and facilitating highly specific delivery of doxorubicin to tumor cells.
Applications
These PEGylated liposomes are particularly useful in cancer treatments that require the targeted delivery of doxorubicin. The azide group reacts with alkyne-functionalized molecules through click chemistry, enhancing the specificity and efficacy of the drug in tumor tissues.
Benefits
The Azide-functionalized PEGylated Doxorubicin Liposomes offer enhanced targeting capabilities, reducing off-target effects and improving therapeutic outcomes. This formulation is ideal for therapies that demand precise delivery of chemotherapeutic agents to cancer cells while minimizing systemic toxicity.
Materials Needed:
- Doxorubicin Liposomes - Azide (PEGylated)
- Syringes and needles
- Phosphate-buffered saline (PBS) for dilution
- Dynamic Light Scattering (DLS) instrument for particle size analysis
- Refrigeration storage (4°C)
- Gloves and appropriate PPE
Procedure:
1. Preparation:
- Ensure that the Doxorubicin Liposomes - Azide (PEGylated) are stored at the recommended temperature (typically 4°C) before use.
- Prepare the necessary dosage based on the experimental or clinical requirements.
2. Conjugation with Targeting Ligands:
- If needed, conjugate the Azide-functionalized liposomes with the desired alkyne-containing targeting ligand before dilution. Follow the specific conjugation protocol for optimal results.
3. Dilution:
- Prior to administration or experimental use, dilute the liposomes in PBS to the desired concentration. Use sterile techniques to avoid contamination.
4. Administration (for research purposes):
- Administer the diluted liposomal solution to the appropriate animal model or in vitro system. The dosage will depend on the experimental design.
5. Particle Size Analysis:
- Use DLS to measure the size distribution of the liposomes. This step is crucial to ensure the consistency of the formulation and to detect any aggregation.
6. Quality Control:
- Assess the encapsulation efficiency and drug release profile of the liposomes using appropriate analytical techniques such as HPLC or UV-Vis spectroscopy.
7. Storage:
- Store the prepared Doxorubicin Liposomes - Azide (PEGylated) at 4°C. Avoid repeated freeze-thaw cycles to maintain stability.
Specifications
| Specification | Details |
|---|---|
| Active Ingredient | Doxorubicin Hydrochloride |
| Lipid Composition | Phosphatidylcholine, Cholesterol, DSPE-PEG (Azide) |
| Liposomal Size | 80-100 nm |
| Encapsulation Efficiency | >90% |
| PEGylation Type | DSPE-PEG (Azide) |
| Storage Temperature | 4°C |
| Usage | Single-use, research and clinical applications |
| Applications | Cancer therapy, targeted drug delivery research |
Doxorubicin Liposomes - Azide (PEGylated) are engineered for specific targeting of cancer cells using bioorthogonal click chemistry. The Azide group reacts with alkyne-functionalized ligands, allowing for precise attachment of targeting molecules and enhanced delivery of doxorubicin to the tumor site, minimizing off-target effects.
Apurun ensures secure shipping of Doxorubicin Liposomes - Azide (PEGylated), with temperature-controlled packaging to maintain product integrity. Standard shipping times apply, with options for expedited delivery available upon request.
Store Doxorubicin Liposomes - Azide (PEGylated) at 4°C. Protect from light and avoid repeated freeze-thaw cycles to maintain efficacy. Proper handling procedures should be followed to ensure safety and product quality.
