General Information
Doxorubicin Liposomes - Cyanur (PEGylated) are advanced drug delivery systems designed for targeted cancer therapy. The cyanuric chloride (Cyanur) functional group attached to the PEGylated liposomes allows for the covalent attachment of specific targeting ligands, enabling precise delivery of doxorubicin to tumor cells.
Applications
These PEGylated liposomes are used in cancer treatments where precise targeting of doxorubicin is critical. The Cyanur group facilitates conjugation with antibodies, peptides, or other targeting molecules, enhancing the specificity and efficacy of the drug within tumor tissues.
Benefits
The Cyanur-functionalized PEGylated Doxorubicin Liposomes provide improved targeting capabilities, reducing off-target effects and enhancing therapeutic outcomes. This formulation is ideal for therapies requiring precise delivery of chemotherapeutic agents to cancer cells while minimizing systemic toxicity.
Materials Needed:
- Doxorubicin Liposomes - Cyanur (PEGylated)
- Syringes and needles
- Phosphate-buffered saline (PBS) for dilution
- Dynamic Light Scattering (DLS) instrument for particle size analysis
- Refrigeration storage (4°C)
- Gloves and appropriate PPE
Procedure:
1. Preparation:
- Ensure that the Doxorubicin Liposomes - Cyanur (PEGylated) are stored at the recommended temperature (typically 4°C) before use.
- Prepare the necessary dosage based on the experimental or clinical requirements.
2. Conjugation with Targeting Ligands:
- If needed, conjugate the Cyanur-functionalized liposomes with the desired targeting ligand (e.g., antibody or peptide) before dilution. Follow the specific conjugation protocol for optimal results.
3. Dilution:
- Prior to administration or experimental use, dilute the liposomes in PBS to the desired concentration. Use sterile techniques to avoid contamination.
4. Administration (for research purposes):
- Administer the diluted liposomal solution to the appropriate animal model or in vitro system. The dosage will depend on the experimental design.
5. Particle Size Analysis:
- Use DLS to measure the size distribution of the liposomes. This step is crucial to ensure the consistency of the formulation and to detect any aggregation.
6. Quality Control:
- Assess the encapsulation efficiency and drug release profile of the liposomes using appropriate analytical techniques such as HPLC or UV-Vis spectroscopy.
7. Storage:
- Store the prepared Doxorubicin Liposomes - Cyanur (PEGylated) at 4°C. Avoid repeated freeze-thaw cycles to maintain stability.
Specifications
| Specification | Details |
|---|---|
| Active Ingredient | Doxorubicin Hydrochloride |
| Lipid Composition | Phosphatidylcholine, Cholesterol, DSPE-PEG (Cyanur) |
| Liposomal Size | 80-100 nm |
| Encapsulation Efficiency | >90% |
| PEGylation Type | DSPE-PEG (Cyanur) |
| Storage Temperature | 4°C |
| Usage | Single-use, research and clinical applications |
| Applications | Cancer therapy, targeted drug delivery research |
Doxorubicin Liposomes - Cyanur (PEGylated) are engineered to allow for specific targeting of cancer cells by conjugating with ligands through the Cyanur group. The PEGylation increases circulation time and reduces recognition by the immune system, enhancing the delivery of doxorubicin to the tumor site while minimizing off-target effects.
Apurun ensures secure shipping of Doxorubicin Liposomes - Cyanur (PEGylated), with temperature-controlled packaging to maintain product integrity. Standard shipping times apply, with options for expedited delivery available upon request.
Store Doxorubicin Liposomes - Cyanur (PEGylated) at 4°C. Protect from light and avoid repeated freeze-thaw cycles to maintain efficacy. Proper handling procedures should be followed to ensure safety and product quality.
