General Information
Fluorescent Liposomes with DOPC, DOPS, and Cholesterol are specialized liposomal formulations that incorporate fluorescent dyes for tracking and imaging purposes. These liposomes are composed of DOPC (Dioleoylphosphatidylcholine), DOPS (Dioleoylphosphatidylserine), and Cholesterol, providing a balanced mix of lipids that mimic biological membranes. The addition of fluorescent markers allows these liposomes to be used in a variety of applications, including cellular uptake studies, biodistribution tracking, and live-cell imaging.
Applications
These fluorescent liposomes are highly effective in research fields such as drug delivery, cellular imaging, and pharmacokinetics. Their fluorescent properties enable real-time tracking of liposomal distribution and interaction with cells, tissues, and biological systems.
Benefits
Fluorescent Liposomes with DOPC, DOPS, and Cholesterol offer the ability to visually monitor liposomal behavior in biological environments, providing critical insights into the mechanisms of drug delivery and cellular uptake. This technology is essential for the development of targeted therapies and the optimization of liposomal formulations.
Materials Needed:
- Fluorescent Liposomes (DOPC:DOPS:Cholesterol)
- Phosphate-buffered saline (PBS) for dilution
- Fluorescence microscope or imaging system
- Dynamic Light Scattering (DLS) instrument for particle size analysis
- Refrigeration storage (4°C)
- Gloves and appropriate PPE
Procedure:
1. Preparation:
- Ensure that the Fluorescent Liposomes are stored at the recommended temperature (typically 4°C) before use.
- Prepare the necessary dosage based on the experimental or clinical requirements.
2. Dilution:
- Prior to administration or experimental use, dilute the liposomes in PBS to the desired concentration. Use sterile techniques to avoid contamination.
3. Administration (for research purposes):
- Administer the diluted Fluorescent Liposomes to the appropriate cell culture or animal model. The dosage and method of administration will depend on the experimental design.
4. Imaging and Detection:
- Use a fluorescence microscope or imaging system to monitor the distribution and uptake of the Fluorescent Liposomes in real-time. Adjust the settings to match the specific fluorescent marker used.
5. Quality Control:
- Use DLS and other analytical methods to confirm the size and stability of the liposomes. Ensure that the fluorescence intensity is consistent with experimental needs.
6. Storage:
- Store any remaining Fluorescent Liposomes at 4°C. Avoid repeated freeze-thaw cycles to maintain stability and fluorescence intensity.
Specifications
| Specification | Details |
|---|---|
| Lipid Composition | DOPC:DOPS:Cholesterol (60:20:20) |
| Liposomal Size | 100-150 nm (can be customized) |
| Fluorescent Marker | Varies depending on application (e.g., FITC, Rhodamine) |
| Storage Temperature | 4°C |
| Usage | Single-use, research and clinical applications |
| Applications | Cellular imaging, drug delivery research, biodistribution tracking |
Fluorescent Liposomes composed of DOPC, DOPS, and Cholesterol are engineered for advanced imaging and tracking applications. The lipid composition mimics biological membranes, providing a stable platform for the incorporation of fluorescent markers. These liposomes are ideal for real-time monitoring of liposomal distribution, cellular uptake, and interactions with biological tissues, making them invaluable in drug delivery research and diagnostics. The choice of fluorescent marker can be tailored to specific experimental needs, ensuring compatibility with various imaging systems.
Apurun ensures secure shipping of Fluorescent Liposomes with DOPC, DOPS, and Cholesterol, with temperature-controlled packaging to maintain product integrity. Standard shipping times apply, with options for expedited delivery available upon request.
Store Fluorescent Liposomes at 4°C. Protect from light to preserve fluorescence intensity, and avoid repeated freeze-thaw cycles to maintain efficacy. Proper handling procedures should be followed to ensure safety and product quality.
