General Information
PG-Based Lyophilized Liposomes are specialized liposomal formulations that incorporate Phosphatidylglycerol (PG) as a key lipid component. These liposomes have undergone lyophilization (freeze-drying), which allows them to be stored in a dry, stable form and reconstituted as needed. The inclusion of PG enhances the liposomes' ability to interact with biological membranes, making them suitable for various applications in drug delivery, vaccine development, and more.
Applications
PG-Based Lyophilized Liposomes are highly effective in fields such as drug delivery, vaccine formulation, and therapeutic research. Their lyophilized form extends shelf life, making them convenient for long-term storage and transport without compromising their structural integrity. Upon reconstitution, they can be used in various in vitro and in vivo studies.
Benefits
These liposomes offer the benefits of enhanced stability and extended shelf life due to lyophilization, combined with the functional properties of PG for targeted delivery and interaction with biological membranes. This makes them a valuable tool in pharmaceutical research and development.
Materials Needed:
- PG-Based Lyophilized Liposomes
- Sterile water or buffer solution for reconstitution
- Phosphate-buffered saline (PBS) for further dilution
- Dynamic Light Scattering (DLS) instrument for particle size analysis
- Refrigeration storage (2-8°C)
- Gloves and appropriate PPE
Procedure:
1. Reconstitution:
- Add sterile water or buffer solution to the lyophilized liposomes. The volume of liquid should be based on the desired final concentration.
- Gently swirl or vortex the vial until the liposomes are fully reconstituted. Avoid vigorous shaking to prevent foaming or damage to the liposomes.
2. Dilution:
- After reconstitution, further dilute the liposomes in PBS if necessary to achieve the desired working concentration.
3. Administration (for research purposes):
- Administer the reconstituted liposomes to the appropriate cell culture or animal model. The dosage and method of administration will depend on the experimental design.
4. Quality Control:
- Use DLS and other analytical methods to confirm the size and stability of the reconstituted liposomes. Ensure that the liposomal structure has been preserved post-lyophilization.
5. Storage:
- Store any remaining reconstituted liposomes at 2-8°C. For long-term storage, it is recommended to keep the liposomes in their lyophilized form.
Specifications
| Specification | Details |
|---|---|
| Lipid Composition | PG:DOPC:Cholesterol (20:50:30) |
| Liposomal Size | 100-150 nm (can be customized) |
| Lyophilization Process | Freeze-drying with cryoprotectants to preserve structure |
| Storage Temperature | 2-8°C for reconstituted, room temperature for lyophilized form |
| Usage | Single-use, research and clinical applications |
| Applications | Drug delivery, vaccine formulation, therapeutic research |
PG-Based Lyophilized Liposomes are designed to maintain their structural integrity and functionality through the lyophilization process. The use of Phosphatidylglycerol (PG) enhances their ability to interact with biological membranes, making them ideal for targeted delivery applications. Upon reconstitution, these liposomes retain their original size and characteristics, allowing for consistent performance in drug delivery and therapeutic studies. The lyophilization process, combined with the lipid composition, provides a stable and versatile platform for various biomedical applications.
Apurun ensures secure shipping of PG-Based Lyophilized Liposomes, with temperature-controlled packaging to maintain product integrity. Lyophilized liposomes can be shipped at ambient temperatures, while reconstituted liposomes should be shipped with cold packs. Standard shipping times apply, with options for expedited delivery available upon request.
Store PG-Based Lyophilized Liposomes at room temperature in their lyophilized form. After reconstitution, store the liposomes at 2-8°C. Protect from light and avoid repeated freeze-thaw cycles to maintain efficacy. Proper handling procedures should be followed to ensure safety and product quality.
