General Information
Phosphatidylcholine (PC) Based Lyophilized Liposomes are specialized liposomal formulations that incorporate phosphatidylcholine, a phospholipid commonly found in cell membranes. These liposomes have undergone lyophilization (freeze-drying), allowing them to be stored in a stable, dry form and reconstituted when needed. Phosphatidylcholine is known for its role in maintaining cell membrane integrity and facilitating the delivery of therapeutic agents, making these liposomes particularly useful in drug delivery, cosmetic formulations, and nutritional supplements.
Applications
Phosphatidylcholine-Based Lyophilized Liposomes are widely used in pharmaceuticals, cosmetics, and nutraceuticals. Their lyophilized form ensures long-term stability, making them convenient for storage and transport. Upon reconstitution, these liposomes can be utilized in both in vitro and in vivo studies, providing an effective delivery system for bioactive compounds, including drugs, vitamins, and other therapeutic agents.
Benefits
These liposomes offer the benefits of extended shelf life and stability due to lyophilization, combined with the membrane-stabilizing properties of phosphatidylcholine. This makes them an excellent choice for applications that require stable and effective lipid bilayers, such as in drug delivery, cosmetics, and nutritional formulations.
Materials Needed:
- Phosphatidylcholine (PC) Based Lyophilized Liposomes
- Sterile water or buffer solution for reconstitution
- Phosphate-buffered saline (PBS) for further dilution
- Dynamic Light Scattering (DLS) instrument for particle size analysis
- Refrigeration storage (2-8°C)
- Gloves and appropriate PPE
Procedure:
1. Reconstitution:
- Add sterile water or buffer solution to the lyophilized liposomes. The volume of liquid should be based on the desired final concentration.
- Gently swirl or vortex the vial until the liposomes are fully reconstituted. Avoid vigorous shaking to prevent foaming or damage to the liposomes.
2. Dilution:
- After reconstitution, further dilute the liposomes in PBS if necessary to achieve the desired working concentration.
3. Administration (for research purposes):
- Administer the reconstituted liposomes to the appropriate cell culture or animal model. The dosage and method of administration will depend on the experimental design.
4. Quality Control:
- Use DLS and other analytical methods to confirm the size and stability of the reconstituted liposomes. Ensure that the liposomal structure has been preserved post-lyophilization.
5. Storage:
- Store any remaining reconstituted liposomes at 2-8°C. For long-term storage, it is recommended to keep the liposomes in their lyophilized form.
Specifications
| Specification | Details |
|---|---|
| Lipid Composition | Phosphatidylcholine (PC):Cholesterol (Varies depending on formulation) |
| Liposomal Size | 100-150 nm (can be customized) |
| Lyophilization Process | Freeze-drying with cryoprotectants to preserve structure |
| Storage Temperature | 2-8°C for reconstituted, room temperature for lyophilized form |
| Usage | Single-use, research and commercial applications |
| Applications | Drug delivery, cosmetics, nutraceuticals, therapeutic research |
Phosphatidylcholine (PC) Based Lyophilized Liposomes are engineered for applications where membrane stability and integrity are essential. Phosphatidylcholine, as a key component of cell membranes, contributes to the structural integrity and fluidity of the liposomal bilayer, making these liposomes suitable for delivering active ingredients in pharmaceutical, cosmetic, and nutritional applications. Upon reconstitution, these liposomes retain their structural integrity, offering consistent performance in delivering bioactive compounds. The lyophilization process ensures long-term stability, making them a reliable choice for formulations that require extended shelf life and robust delivery systems.
Apurun ensures secure shipping of Phosphatidylcholine (PC) Based Lyophilized Liposomes, with temperature-controlled packaging to maintain product integrity. Lyophilized liposomes can be shipped at ambient temperatures, while reconstituted liposomes should be shipped with cold packs. Standard shipping times apply, with options for expedited delivery available upon request.
Store Phosphatidylcholine (PC) Based Lyophilized Liposomes at room temperature in their lyophilized form. After reconstitution, store the liposomes at 2-8°C. Protect from light and avoid repeated freeze-thaw cycles to maintain efficacy. Proper handling procedures should be followed to ensure safety and product quality.
