General Information
PS-Based Lyophilized Liposomes are specialized liposomal formulations that incorporate Phosphatidylserine (PS) as a key lipid component. These liposomes have undergone lyophilization (freeze-drying), allowing them to be stored in a stable, dry form and reconstituted as needed. PS is known for its role in cellular processes such as apoptosis and signaling, making these liposomes particularly useful in therapeutic research, drug delivery, and cellular studies.
Applications
PS-Based Lyophilized Liposomes are used in various research fields, including drug delivery, vaccine development, and cell biology studies. Their lyophilized form ensures long-term stability, making them convenient for storage and transport. Upon reconstitution, they retain their structural integrity and can be used in both in vitro and in vivo experiments.
Benefits
These liposomes offer the benefits of extended shelf life and stability due to lyophilization, along with the functional properties of PS for enhanced interaction with biological membranes. This makes them an excellent choice for researchers looking to study cell signaling, apoptosis, or deliver therapeutic agents effectively.
Materials Needed:
- PS-Based Lyophilized Liposomes
- Sterile water or buffer solution for reconstitution
- Phosphate-buffered saline (PBS) for further dilution
- Dynamic Light Scattering (DLS) instrument for particle size analysis
- Refrigeration storage (2-8°C)
- Gloves and appropriate PPE
Procedure:
1. Reconstitution:
- Add sterile water or buffer solution to the lyophilized liposomes. The volume of liquid should be based on the desired final concentration.
- Gently swirl or vortex the vial until the liposomes are fully reconstituted. Avoid vigorous shaking to prevent foaming or damage to the liposomes.
2. Dilution:
- After reconstitution, further dilute the liposomes in PBS if necessary to achieve the desired working concentration.
3. Administration (for research purposes):
- Administer the reconstituted liposomes to the appropriate cell culture or animal model. The dosage and method of administration will depend on the experimental design.
4. Quality Control:
- Use DLS and other analytical methods to confirm the size and stability of the reconstituted liposomes. Ensure that the liposomal structure has been preserved post-lyophilization.
5. Storage:
- Store any remaining reconstituted liposomes at 2-8°C. For long-term storage, it is recommended to keep the liposomes in their lyophilized form.
Specifications
| Specification | Details |
|---|---|
| Lipid Composition | PS:DOPC:Cholesterol (20:50:30) |
| Liposomal Size | 100-150 nm (can be customized) |
| Lyophilization Process | Freeze-drying with cryoprotectants to preserve structure |
| Storage Temperature | 2-8°C for reconstituted, room temperature for lyophilized form |
| Usage | Single-use, research and clinical applications |
| Applications | Drug delivery, vaccine development, cell signaling research, apoptosis studies |
PS-Based Lyophilized Liposomes are designed to maintain their structural integrity and functionality through the lyophilization process. Phosphatidylserine (PS) plays a critical role in cell signaling, particularly in processes like apoptosis, making these liposomes particularly useful for studies focused on these pathways. Upon reconstitution, these liposomes retain their original size and characteristics, allowing for consistent performance in research and therapeutic applications. The lyophilization process, combined with the lipid composition, provides a stable and versatile platform for various biomedical applications.
Apurun ensures secure shipping of PS-Based Lyophilized Liposomes, with temperature-controlled packaging to maintain product integrity. Lyophilized liposomes can be shipped at ambient temperatures, while reconstituted liposomes should be shipped with cold packs. Standard shipping times apply, with options for expedited delivery available upon request.
Store PS-Based Lyophilized Liposomes at room temperature in their lyophilized form. After reconstitution, store the liposomes at 2-8°C. Protect from light and avoid repeated freeze-thaw cycles to maintain efficacy. Proper handling procedures should be followed to ensure safety and product quality.
