General Information
HSPC:Cholesterol Surface Reactive Liposomes are specialized liposomal formulations that incorporate Hydrogenated Soy Phosphatidylcholine (HSPC) and Cholesterol. These liposomes are surface reactive, designed for specific applications where surface modification or interaction with biological molecules is required. The surface reactivity makes them ideal for targeted drug delivery, bioconjugation, and other advanced biomedical applications.
Applications
These surface reactive liposomes are particularly effective in drug delivery systems that require functionalization, targeting, or interaction with specific biomolecules. They are widely used in the development of targeted therapies, diagnostic tools, and in vitro studies where surface modification is critical.
Benefits
HSPC:Cholesterol Surface Reactive Liposomes offer enhanced stability and the ability to conjugate with various ligands, antibodies, or other targeting moieties. This flexibility allows for precise delivery and targeting in therapeutic applications, making them a valuable tool in the development of advanced drug delivery systems and diagnostics.
Materials Needed:
- HSPC:Cholesterol Surface Reactive Liposomes
- Phosphate-buffered saline (PBS) for dilution
- Dynamic Light Scattering (DLS) instrument for particle size analysis
- Refrigeration storage (4°C)
- Appropriate reagents for surface modification (e.g., ligands, antibodies)
- Gloves and appropriate PPE
Procedure:
1. Preparation:
- Ensure that the HSPC:Cholesterol Surface Reactive Liposomes are stored at the recommended temperature (typically 4°C) before use.
- Prepare the necessary dosage based on the experimental or clinical requirements.
2. Surface Modification:
- Mix the liposomes with the desired reagents (e.g., ligands, antibodies) to achieve the desired surface modification. The reaction conditions will depend on the specific chemistry involved.
3. Dilution:
- After surface modification, dilute the liposomes in PBS to the desired concentration for further use. Use sterile techniques to avoid contamination.
4. Administration (for research purposes):
- Administer the modified liposomes to the appropriate cell culture or animal model. The dosage and method of administration will depend on the experimental design.
5. Quality Control:
- Use DLS and other analytical methods to confirm the size and stability of the modified liposomes. Assess the efficiency of surface modification using appropriate assays.
6. Storage:
- Store any remaining modified liposomes at 4°C. Avoid repeated freeze-thaw cycles to maintain stability and functionality.
Specifications
| Specification | Details |
|---|---|
| Lipid Composition | HSPC:Cholesterol (55:45) |
| Liposomal Size | 100-150 nm (can be customized) |
| Surface Reactivity | Reactive towards ligands, antibodies, or other functional groups |
| Storage Temperature | 4°C |
| Usage | Single-use, research and clinical applications |
| Applications | Targeted drug delivery, bioconjugation, diagnostics |
HSPC:Cholesterol Surface Reactive Liposomes are engineered for advanced drug delivery and diagnostic applications where surface modification is essential. The combination of HSPC and cholesterol provides stability, while the surface reactivity allows for the attachment of various functional groups, including ligands, antibodies, or other targeting moieties. This capability makes them suitable for targeted therapies and precision medicine, where specific cell targeting and drug delivery are critical.
Apurun ensures secure shipping of HSPC:Cholesterol Surface Reactive Liposomes, with temperature-controlled packaging to maintain product integrity. Standard shipping times apply, with options for expedited delivery available upon request.
Store HSPC:Cholesterol Surface Reactive Liposomes at 4°C. Protect from light and avoid repeated freeze-thaw cycles to maintain efficacy. Proper handling procedures should be followed to ensure safety and product quality.
