General Information
Tracer Liposomes are specialized liposomal formulations designed for tracking and tracing in biological systems. These liposomes are labeled with detectable tracers, such as fluorescent dyes or radioactive isotopes, to monitor their distribution and uptake in vivo and in vitro. The following lipid compositions are used:
- DOPG:DOPC:Cholesterol (20:50:30)
- DOPG:DOPC:Cholesterol (20:50:30)
- DOPC:Cholesterol (70:30)
- DOTAP:DOPC:Cholesterol (5:65:30)
Applications
Tracer Liposomes are used in drug delivery research, pharmacokinetic studies, biodistribution analyses, and more. The labeling of liposomes with detectable tracers allows for precise monitoring and imaging of their behavior in biological systems.
Benefits
Tracer Liposomes enable real-time monitoring of therapeutic delivery and distribution, providing valuable insights into the efficiency of drug delivery systems. This technology is crucial for developing targeted therapies and optimizing liposomal formulations.
Materials Needed:
- Tracer Liposomes (labeled with desired tracer)
- Phosphate-buffered saline (PBS) for dilution
- Dynamic Light Scattering (DLS) instrument for particle size analysis
- Fluorescence or radioactivity detection equipment
- Refrigeration storage (4°C)
- Gloves and appropriate PPE
Procedure:
1. Preparation:
- Ensure that the Tracer Liposomes are stored at the recommended temperature (typically 4°C) before use.
- Prepare the necessary dosage based on the experimental or clinical requirements.
2. Dilution:
- Prior to administration or experimental use, dilute the liposomes in PBS to the desired concentration. Use sterile techniques to avoid contamination.
3. Administration (for research purposes):
- Administer the diluted Tracer Liposomes to the appropriate cell culture or animal model. The dosage and method of administration will depend on the experimental design.
4. Detection and Analysis:
- Use appropriate detection equipment (e.g., fluorescence microscopy, gamma counter) to monitor the distribution and uptake of the Tracer Liposomes in the biological system.
5. Quality Control:
- Assess the stability and integrity of the Tracer Liposomes using DLS and other relevant techniques to ensure accurate tracing results.
6. Storage:
- Store any remaining Tracer Liposomes at 4°C. Avoid repeated freeze-thaw cycles to maintain stability and tracer integrity.
Specifications
| Specification | Details |
|---|---|
| Lipid Composition | DOPG:DOPC:Cholesterol (20:50:30), DOPG:DOPC:Cholesterol (20:50:30), DOPC:Cholesterol (70:30), DOTAP:DOPC:Cholesterol (5:65:30) |
| Liposomal Size | 100-150 nm (can be customized) |
| Tracer Type | Fluorescent dye, radioactive isotope, or other markers |
| Storage Temperature | 4°C |
| Usage | Single-use, research and clinical applications |
| Applications | Biodistribution studies, drug delivery research, imaging |
Tracer Liposomes are engineered to facilitate the tracking and tracing of liposomes in biological systems. By incorporating detectable tracers, such as fluorescent dyes or radioactive isotopes, researchers can monitor the behavior of liposomes in real-time, gaining insights into their distribution, uptake, and overall efficiency as drug delivery vehicles. This technology is essential for optimizing liposomal formulations and developing targeted therapies.
Apurun ensures secure shipping of Tracer Liposomes, with temperature-controlled packaging to maintain product integrity. Standard shipping times apply, with options for expedited delivery available upon request.
Store Tracer Liposomes at 4°C. Protect from light (if labeled with fluorescent tracers) and avoid repeated freeze-thaw cycles to maintain efficacy. Proper handling procedures should be followed to ensure safety and product quality.